Isagi Yoichi, a first-year forward, loses his chance for the Nationals because he has passed the ball to his fellow player in front of the goal. That's when he receives an invitation for Blue Lock. It's a facility to train one world's greatest striker out of 300 high-school-aged forwards so that Japan can win the World Cup.

Rin chooses to add Isagi to his team, and together with Bachira they pass the second selection. Waiting for them on the other side is a 5-on-5 match against a team of world-class players. Thanks to Isagi, Bachira, and Rin's coordinated play, they manage to score the first goal, but they soon find themselves overwhelmed by the opposing team's superior skill and ability. Despite this, they desperately keep fighting until the last second, unleashing their own "egos." As a result, the "Blue Lock" project, aiming to produce the world's greatest striker, enters a new phase.