Fresh off their monumental win at Sectionals, New Directions is on top of the world. With an extra spring in their step, they get to work on new numbers for regionals. But when Rachel and Finn’s budding romance hits a bump in the road after she becomes involved with a competitor, Jesse St. James, it threatens to derail the entire club. Mr. Schuester pays a visit to Shelby Corcoran, coach of Vocal Adrenaline, to confirm Jesse’s motives. Meanwhile, Will is newly single, but is he ready to take his relationship with Emma to the next level? And to top it all off, Sue Sylvester returns from her condo in Boca with a renewed focus on destroying Will and disbanding the glee club.

Mr. Schuester is concerned that the girls in the glee club are being disrespected and bullied by the guys. Looking to Madonna and her musical message of equality as inspiration, he challenges the entire team to choose Madonna songs as their next assignment in the hopes that the girls embrace her strength, independence and confidence and that the guys will see the error of their misogynistic ways. The glee club takes the assignment to heart as they perform some of Madonna’s greatest hits, including “Express Yourself” and “Like A Prayer.” Meanwhile, Kurt and Mercedes give Sue a vote of confidence with a makeover that is definitely in “Vogue”.

When Kurt sets his dad, Burt, up with an unexpected partner his plan for a nuclear family may backfire. Sue demands that the Cheerios! go on a crazy diet in preparation for a big interview with “Splits” magazine and April Rhodes returns to save Will and the glee club.

Mr. Schuester starts an investigation when a salacious list about members of the glee club circulates the halls of McKinley High. Meanwhile, Sue is mortified when a video of her performing the Olivia Newton-John classic "Physical" surfaces

Mr. Schuester’s former high school nemesis, Bryan Ryan, causes trouble for the glee club. Meanwhile, Rachel struggles with a life-long personal issue, and Artie’s dreams take him on an adventure.

The glee club gets some news that’s sure to rattle a few nerves. Meanwhile, Quinn connects with her “funky” side with a little help from Mercedes, and Will gets revenge on Sue.

The moment New Directions has been waiting for has finally arrived: it’s time for Regionals! But when Sue pulls a fast one on Will and the glee clubbers, their chances at the title could be compromised. Meanwhile, Quinn’s life changes forever.

When the entertainment for prom falls through, Principal Figgins asks New Directions to perform at the dance much to prom coordinator Sue’s dismay. Things get heated as the competition for prom king and queen draws closer, and Jessie St. James returns.

New Directions continues to strategize their set list for Nationals with a little help from Jessie St. James. Meanwhile, an unexpected loss rocks the world of one of McKinley’s own.

New Directions heads to Nationals to compete in the ultimate singing showdown.

The Glee Club does disco in a tribute to Saturday Night Fever.

The glee club travels to Chicago, Illinois, to compete in Nationals against the best show choirs in the country, including Vocal Adrenaline, for celebrity judges including Lindsay Lohan and Perez Hilton.

New Directions and the Warblers go head-to-head at sectionals. Meanwhile, Rachel must choose between returning to NYADA and doing another Broadway show with her past love, Jesse St. James.

All of Will’s hard work and dedication to the Glee Club and to fostering the talents and dreams of his students pays off in a major way, and everyone shares in the feeling of joy that permeates the school as teachers and students reflect on their shared journey. Old rifts and wounds are healed and new sights are set on a promising future. Everyone seems to be ending up exactly as expected and right where they belong… along with more than a few unexpected surprises.