Three suburban housewives on a girls' weekend in Miami are the prime suspects when the hotel's handsome pool boy is found murdered. When Horatio is tipped off that his team is facing a surprise efficiency review, he must trust Calleigh, Delko and Ryan to work together to find the killer. However, when they independently follow the evidence, each discovers that their analysis points to a different killer.

A convicted serial killer escapes after sabotaging an airplane that was flying him from New York to Miami, where he allegedly buried a body. After fleeing the crash site, the man goes on a killing spree and abducts a college student. Mac Taylor arrives to help Horatio track the killer since Mac originally arrested the man in New York.

The crossover concludes on CSI: New York S02E07 Manhattan Manhunt (II).