In the same frame-story format, Hader and Samberg apologize to Lorne Michaels for the original Laser Cats! claiming to understand where they went wrong: not enough politics. They then introduce Laser Cats! 2, based on the same premise, now set in the Iraq War which has gone nuclear and caused the feline mutations. This time, Dr. Scientist (Jake Gyllenhaal) has stolen the cure that turns Laser Cats back into regular cats. Jorma Taccone appears in the opening credits for Laser Cats! 2 (Which is the same as Laser Cats!), and Fred Armisen and Amy Poehler are the scientists who created the cure.

Samberg sings a love song for Mahmoud Ahmadinejad (Armisen), along with Adam Levine from Maroon 5, sampling "Avril 14th" by Aphex Twin. Jake Gyllenhaal has a cameo.