Reluctant witch Diana Bishop and vampire Matthew Clairmont seek refuge in the fascinating and treacherous world of Elizabethan London. There, they must find a powerful witch to help Diana control her magic and search for the elusive Book of Life.

As she continues her search for a mentor, Diana meets wealthy alchemist Mary Sidney. London's vampire ruler demands fealty from Diana and Matthew as he is unnerved by their sudden arrival in the City.

In the present, Domenico makes a disturbing discovery in Oxford. Back in 1590, Matthew must beg for forgiveness from Father Hubbard.

Matthew and Diana go to Bohemia to search for Edward Kelley and the Book of Life. There, they encounter the strange Emperor Rudolf II.

As Diana and Matthew return to London, Diana learns news that could change everything. The Queen holds Matthew to account.

The second series reaches a dramatic conclusion. Realising that she must return to the present, Diana aims to complete her training.