Radha and Jayalakshmi, popularly known as Radha Jayalakshmi (Tamil: ராதா ஜெயலட்சுமி), were an Indian Carnatic music vocalist duo as well as playback singers in films in the 1940s and 1950s. They later became teachers and trained notable Carnatic music singers. Jayalakshmi was the playback singer of the duo, but was credited as Radha Jayalakshmi in the cine field. Radha was her cousin and singing partner on stage performances. They were early vocalists in the duo singing trend in Carnatic music which started in the 1950s and includes performers like Bombay Sisters and Soolamangalam Sisters. In recent times, the trend has been continued by popular Carnatic music singers like Priya Sisters, their disciples, and Ranjani Gayatri.

The duo was awarded the 1981 Sangeet Natak Akademi Award in Carnatic Music – Vocal, given by the Sangeet Natak Akademi, India's National Academy for Music, Dance and Drama Vidushi Jayalakshmi died in Chennai on 27 May 2014.